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Applied Science Private University

Volume Volume 18, 2024 · Issue 1 · Open Access · Double-blind Peer-review

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Hypothesis

In-Vitro Study on the Antibacterial and Antifungal Effects of Different Aqueous and Alcoholic Extracts from Curcuma Longa Rhizomes

Mohammad A. A. AL-Najjar1, Feras Darwish Elhajji2, Ruaa R Alalwany3, Saleh Alnaji4, Zaid Alherbawi4, Sawsan Abu Jamma’ah4 & Shatha Alshaer2

1 Department of Pharmaceutical Sciences and Pharmaceutics, Faculty of Pharmacy, Applied Science Private University, Amman, Jordan.

2 Department of Clinical Pharmacy and Therapeutics, Faculty of Pharmacy, Applied Science Private University, Amman, Jordan.

3 Department of Pharmaceutical Sciences and Pharmaceutics, Faculty of Pharmacy, Applied Science Private University, Amman – Jordan.

* Corresponding author: Mohammad A. A. AL-Najjar ([email protected])

Published: 30 Jun 2024

https://doi.org/10.35192/jjoas-n.v18i1.1569
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Abstract

Background: Turmeric (Curcuma longa) has been popular since ancient times in both nutritional and medicinal recipes. In modern science, studies have proven its effectiveness in inhibiting the growth of some pathogenic microorganisms, but this effectiveness depends on several factors, including the type of extraction solvents used to extract the active substances from turmeric and the method of extraction. This study investigates the antimicrobial effect of different turmeric extracts using the minimum inhibitory concentration (MIC) method.

Methods: Forty grams of dried turmeric rhizome powder were soaked in 400 ml of each solvent (distilled water, ethanol, and ethyl acetate) separately for one week. Each mixture was filtered, and the remaining solvent was evaporated. The minimum inhibitory concentration (MIC) for each crude extract was measured using microdilution broth techniques against Staphylococcus aureus (ATCC 25923), Escherichia coli (ATCC 14169), and Candida albicans (ATCC 10231).

Results: The MIC values for the aqueous turmeric extract against the two tested bacteria (S. aureus and E. coli) were 0.39±0.07 μg/μl and 1.562±0.33 μg/μl, respectively. For the tested fungus (C. albicans), the MIC was 50±0.05 μg/μl. However, the ethyl acetate turmeric extract inhibited only S. aureus (MIC = 0.39±0.04 μg/μl).

Conclusion: This study revealed the antimicrobial effects of the aqueous turmeric extract against the three tested microorganisms and the antibacterial effect of the ethyl acetate turmeric extract against S. aureus. However, no inhibitory activity was observed for the ethanol turmeric extract against any tested microorganism.

KeywordsTurmeric extractsantimicrobial activityStaphylococcus aureusEscherichia coliCandida albicans

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